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A rapid and specific method for screening, identifying and quantifying 17 sulfonamide antibiotics was developed using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS). After preliminary optimization of the sample preparation process, a one-step sample preparation technique was developed. The crude extracts were directly injected into the UHPLC-MS for screening, a set of 13 sulfonamides were quantified by matrix-matched calibration and the detection limit was found to be between 0.3 and 4 ng/mL. After enzymolysis for 4 h at 60 °C in phosphate-buffered saline at pH 7.0, a total of 28 antibiotics, including 17 sulfonamides, were quantified and the limit of detection (LOD) of the method was between 2 and 50 ng/mL. Finally, 5 sulfonamide antibiotics were quantified in pooled human urine samples, suggesting that this method is appropriate for the screening, identification and quantification of sulfonamide antibiotics in clinical and industrial bio-resources.The N.B.A. trade value has increased, but that’s because it’s tougher to trade. And it is tougher.
Each trade requires more due diligence and leads to more negotiations. No more faxes or e-mails and fewer handshakes. It is harder to do deals in person, too.
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The Milwaukee Bucks can sign Jabari Parker, a consensus national player of the year, to a long-term contract for less than $1 million a year. The salary cap for the 2015